The real-time PCR process including sample and reaction setup, thermal cycling, and fluorescent detection. Focused application software analyzes and interprets experimental results. Depending on the experimental design, the system can even help you select and order real-time PCR reagents online by means of convenient links in the software. The StepOne™ Real-Time PCR System is covered by US patents and corresponding claims in their non-US counterparts, owned by Applied Biosystems. No right is conveyed expressly, by implication, or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods such. REST won't work on a mac or on a windows 7-machine. You can however use the qPCR package implemented in R; this will do different simulations with your data where one is simmilar to the REST approach. The MyGo Pro real-time PCR instrument provides unmatched performance in a convenient format. Novel Full Spectrum Optics deliver 120 optical channels of fluorescence data from every tube in parallel, with no moving parts, for reliable multiplex PCR. Software Downloads StepOne and StepOnePlus Real-Time PCR System NOTE: Prior to downloading any software to a computer used for instrument control/operation, please consult with your Service and Support representative.

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Speed

Mic uses a patented magnetic induction technology to achieve heating and forced airflow for cooling. This means faster heating and cooling times. Back that up with a robust optical system that reads all four channels simultaneously and running multichannel assays has never been quicker.

• Very fast cycling times with 35 cycles possible in less than 25 minutes*
• Instruments are available with either 2 (Green/Yellow) or 4 channels (Green/Yellow/Orange/Red)
• Separate high intensity LED excitation source for each channel (uniform excitation output) and a separate emission filter and detector for each channel

*Assays designed toward cDNA targets with short amplicon sizes and using fast cycling compatible polymerases

Accuracy

Mic’s spinning aluminium rotor gives unsurpassed temperature uniformity during dynamic and static operations. All other block-based cyclers only promise static uniformity, which could lead to inaccurate data, as samples aren’t heated and cooled equally. You might think this level of accuracy requires constant calibration, but Mic’s good to go right out of the box. You don’t need to calibrate – ever.

• Excellent reproducibility between samples and repeatability between runs and instruments ensures the highest level of quantification precision; allowing for the detection of two-fold differences in gene expression levels.
• Fixed optical path with no moving parts means no optical alignment or calibration and no need for a ROX reference dye.

Temperature accuracy : ±0.25ºC, well-to-well temperature uniformity : ±0.05ºC, superior equilibration time uniformity (zero seconds for all wells to reach the same temperature)Class IV SNP (A to T), temperature difference between alleles : <0.1°C

Size

Mic takes up less space on the bench than your lab book. And weighing in at just 2 kg, this is the most portable qPCR cycler on the market. Even four Mic’s stacked together take up less bench space than your current cycler.

• The magnetic induction technology allows for a small instrument footprint to be achieved, by way of its elegant simplicity.
• The tube format uses 0.1 ml strips of four tubes and matching caps, supporting volumes of 10 to 25 µl. A tab on tube 1 ensures correct orientation of tubes into the instrument.
• High speed centrifugation ensures samples spin down at the start of a run, and during a run will remove bubbles and prevent condensation, eliminating the need for bulky heated lids and external centrifuges.

Connectivity

Multiple Mics can be operated from one workstation so 48 becomes 96, and 96 becomes 192. Bluetooth technology means fewer cables too.

• Instruments can communicate via Bluetooth or USB cable. As many as ten instruments can be run from the one PC allowing for up to 480 samples to be run simultaneously.
• Individual runs can be collated into a project and analysed together.
• Installation is plug-and-play.

Software

Next generation qPCR analysis software that is user-friendly and packed with intelligent features. See your results with detailed statistical analysis as soon as your run has completed. Designed to meet MIQE specifications, the Mic software offers you the most up-to-date qPCR analysis.

Projects

Take advantage of Mic’s modular functionality and amazing reproducibility by combining multiple runs from multiple instruments into one analysis. With the ability to combine up to 10 runs you can analyse up to 480 samples at one time. Now there is no need to wait for samples to be batched into one 96 or 384 well run. Complete the runs at the time you need them done and see the results now, not later.

Quantitation

Confidently detect 2 fold differences in gene expression levels. Whether it’s using standard curves for Absolute Quantification or determining gene expression using Relative Quantification through REST, know that the performance you get from the Mic will always ensure the highest level of quantitative precision.

Manganese superoxide dismutase gene (MnSOD)
Eight point, 2x dilution series of human genomic DNA (n = 4 each)
Efficiency = 98% (standard curve method)
R² = 1.00

Extreme quantitative precision

Detect differences within one cycle. When you need to quantify small differences in relative gene expression, Mic will deliver the extreme levels of quantitative precision you need. Especially for bacterial genetics where minor differences in gene expression can multiply into big differences.

A wide linear dynamic range

Down to single digit copies of DNA. Have the power to detect high and low copy numbers of your target. Be it using Absolute Quantification to detect viral loads, or simply determining a PCR efficiency using your standard curve, Mic will deliver the dynamic range you need, accurately and precisely.

10 point, 10x dilution series of Hepatitis B virus (HBV) cDNA template
Starting amount of 3E+09 copies (n = 3 each) over 10 logs
Efficiency = 95% (standard curve method)
R² = 0.99

Amazing repeatability

Ultra tight replicates every time. Be confident in the knowledge that each well is behaving identically to generate qPCR replicate data that is truly repeatable. Our focus on temperature uniformity means you can have confidence in your results whether its quantifying gene expression, determining genotypes, or measuring viral load.

Manganese Superoxide Dismutase (MnSOD) (n = 48)
Standard Deviation = 0.03
Cq Difference = 0.2
Efficiency = 98% (LinRegPCR method)

Outstanding reproducibility

Get the same result across multiple runs and instruments. We build our instruments to perfection so that each one is identical to the next. This means we can reproduce the same result not just across multi-runs, but instruments as well. Now you can combine multiple runs with minimal concern for variation. Inter-run calibrators become more of a quality control measure than a correction tool. And using sophisticated analysis software incorporating intelligent methods such as LinRegPCR, means we can further improve the qPCR reproducibility by minimising human error through analytical automation.

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Human chromosome Y template

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Template amount was 5E+06 copies/µl (n = 30)
3 different instruments and 3 different experiments set up at different times
CV across three instruments = 3%

Reproducibility at low copy number

Duplicate runs easily on different instruments. Mic’s reproducibility even extends to very low copy numbers. Critical in applications such as Relative Quantification, Absolute Quantification and Copy Number Variants (CNV).

KRAS proto-oncogene, exon 2
Template amount was 200 copies/µl human genomic DNA (n = 48)
Two different instruments and three different experiments set up at different times
CV across two instruments and runs = 6%

Fast cycling

Maintain assay performance even at speed. Get high quality data, fast ! Mic’s speed is on par with the fastest instruments on the market. But unlike the competition Mic’s superior temperature uniformity and accuracy means you don’t sacrifice on the quality of your qPCR. Completing runs in under 30 minutes is the new standard with Mic, not the exception.

5 point, 2x dilution series of Hepatitis B virus (HBV) cDNA template
Starting amount of 3E+06 copies (n = 4 each)
Efficiency = 90% (standard curve method) ; R² = 0.99
Time to complete run (including melt) = 26 min

Four colours

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Lowest possible cross talk. If it’s molecular diagnostic (MDx) detection of pathogens, or genotyping using Allelic Discrimination, Mic has highly optimized filter sets to minimize your dye cross talk when multiplexing your real time PCR. With dedicated high powered LED and detectors per channel, detect all four colours in 1 sec during acquisition. No dye colour compensation or dye calibration needed – ever !

Individual channel high power LEDs
Individual channel detectors
Cross talk < 3% across all channels

The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. The StepOnePlus™ Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 4-color optical recording, the StepOnePlus™ Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.

Step One Plus Real Time Pcr Software Download

To download the latest version of the StepOne™ Plus v2.3 software onto your personal computer just follow the link below: http://www.lifetechnologies.com/us/en/home/technical-resources/software-downloads/StepOne-and-StepOnePlus-Real-Time-PCR-System.html

DOM location: 650 Albany Street, Room X-830
Instrument Manager: Matthew Au

Primer Express Software v3.0

The Primer ExpressR Software allows you to design your own primers and probes using TaqManR and SYBR Green I dye chemistries for gene quantitation and allelic discrimination (SNP) real-time PCR applications.

DOM location: 650 Albany Street, Room X-830
Instrument Manager: Lynn Lingyi Deng, Ph.D

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Contact

For further information on our Analytical Services or to schedule a meeting to develop a project, please contact:

Matthew Au AIC Lab Manager 650 Albany Street, Room 830 Boston MA 02118 mau115@bu.edu 617-358-6363

Lynn Deng Director of Analytical Instrumentation Core 650 Albany Street, Room 848 Boston MA 02118 lynndeng@bu.edu 617-358-6516

Return to the Analytical Instrumentation Core page.